Friday, October 29, 2021

The Tarim mummies were not a people or a tradition, but a natural phenomenon.

 NOTE: There will be several images of mummies and dead faces posted here, so if that isn't your cup of tea be aware.

Sorry but not sorry for the snarky 'well ackshually' title gents, but this is a common mistake I see people make. I'll elaborate further below. If by now you haven't heard of the exciting news of the Tarim Mummies, please check out the article right now!

The genomic origins of the Bronze Age Tarim Basin mummies (Click here to read)

Abstract:
The identity of the earliest inhabitants of Xinjiang, in the heart of Inner Asia, and the languages that they spoke have long been debated and remain contentious1. Here we present genomic data from 5 individuals dating to around 3000–2800 BC from the Dzungarian Basin and 13 individuals dating to around 2100–1700 BC from the Tarim Basin, representing the earliest yet discovered human remains from North and South Xinjiang, respectively. We find that the Early Bronze Age Dzungarian individuals exhibit a predominantly Afanasievo ancestry with an additional local contribution, and the Early–Middle Bronze Age Tarim individuals contain only a local ancestry. The Tarim individuals from the site of Xiaohe further exhibit strong evidence of milk proteins in their dental calculus, indicating a reliance on dairy pastoralism at the site since its founding. Our results do not support previous hypotheses for the origin of the Tarim mummies, who were argued to be Proto-Tocharian-speaking pastoralists descended from the Afanasievo1,2 or to have originated among the Bactria–Margiana Archaeological Complex3 or Inner Asian Mountain Corridor cultures4. Instead, although Tocharian may have been plausibly introduced to the Dzungarian Basin by Afanasievo migrants during the Early Bronze Age, we find that the earliest Tarim Basin cultures appear to have arisen from a genetically isolated local population that adopted neighbouring pastoralist and agriculturalist practices, which allowed them to settle and thrive along the shifting riverine oases of the Taklamakan Desert.

If you are a bit more in the know however, you might've heard of this as early as August of this year, like I did. And if so this news isn't all that surprising right now, but when you first heard it it was probably incredibly surprising. It sure was for me!

 I did expect that prior to Indo-European migration to Xinjiang, there would have been populations similar to those in South Siberia and Central Asia (Botai, Kelteminar, Bolshemys etc) inhabiting this region  based on the archaeological records as well as genomic ancestry of the Shirenzigou samples and the maternal haplogroup C4 which has been uncovered in the Tarim and was also found in those populations. 


Wooden anthropomorphic statues from Xiaohe

What I did not expect however, was how genetically isolated these earlier inhabitants were, and the fact that the famous mummies such as the beauty of Loulan and the Xiaohe Princess belonged to these populations.

Crazy right? Especially if you have been keeping up with this for a minute, as an article from 2010 claimed that all the paternal haplogroups were R1a-M198, making them good candidates to be lineages dispersed by early Indo-European peoples. But there might be some issues with that. 

Before the article was published I was kind of able to figure out that the samples labeled as XH and GMG were from Xiaohe and Gumugou respectively, and that some of the Xiaohe samples overlapped with samples from an earlier study from 2021. Thus I was able to figure out the layers which they were attributed to. I also figured that the Dzungaria_EBA samples were probably just Afanasievo given their location and timeframe, and I was correct regarding that.

The supposed R1a samples came from the oldest layers at Xiaohe, yet these newly published samples from Xiaohe also came from the same layers and one younger, neither showing any signs of Western Steppe Herder ancestry. Their only y-dna haplogroup was R1b-PH155. I think that these R1a calls might be incorrect, or alternatively that the samples were attributed to the incorrect layer but I find that a little less likely. 

After the shocking dust settled, it actually dawned onto me how this made things a whole lot clearer. From an archaeological perspective, an Afanasievo > Chemurchek or Abashevo>Sintashta>Andronovo derived origin for these peoples were somewhat strange anyways as there was a very different material culture. But shifts in material cultures are quite common-place so this in itself can be explained away.

One big mystery is why these sites did not contain ceramic pottery, but had these intricate weaved baskets instead. Meanwhile both the Afanasievo and Andronovo had ceramic pottery, as did the Central Asian and Siberian native populations, for thousands of years at that point.These populations were the EHG, EHG/CHG and/or East Asian admixed distant relatives of these Tarim_EMBA populations. Yet there was no pottery at Xiaohe and Gumugou?

The genetic isolated profile might be a clue in this regard. Poterry spread through Siberia during the 6th and 5th millenium b.c, and if these populations separated from their relatives prior to this, they might have missed the mark in regards to this new pottery techniques rapidly spreading through northern Eurasia. Archaeological evidence seems to suggest habitation of the Tarim Basin during that period by foragers, and these may have been the ancestors of the Tarim_EMBA populations as the TMRCA of R1b-PH155 matches up very well with these dates.

Xiaohe weaved basket (reconstruction)

Another clue is that there are no real weapons or warrior-esque burials in these sites. Meanwhile these are regularly featured in Afanasievo, Chemurchek and Andronovo sites (and are a big element of Indo-European cultures as a whole), as well across the south Siberian populations with the Seima-Turbino phenomenon. Yet it isn't present amongst the early Tarim cultures.

These two archaeological discrepancies are very easily explained by the genetic ancestry of the populations, which were not genetically derived from Indo-European speaking peoples.

I have to say that I wasn't impressed with their conclusions for the Siberian and Central Asian populations derived from Neolithic West-Siberian related peoples. It seems the authors are unaware of the existence of EHG/CHG related populations such as those uncovered at Progress and Vonyuchka. Or that these populations migrated to the North Caspian region. And that populations such as Steppe Maykop and Kumsay_EBA more or less seem halfway inbetween WSHG and Progress/Vonyuchka (50/50). Thus whenever there is a steppe-like signal required, it gets attributed to either Yamnaya or Dzungaria_EBA.


But this isn't all that surprising after their "amazing" conclusions that the Shirenzigou samples were Afanasievo-derived, although these proposals are a bit more accurate than what was proposed in 2019. There is a lot of overlapping ancestries involved here and when details are overlooked you can have some incorrect assesments.

The Dzungaria_EBA samples, basically just Xinjiang Afanasievo, are quite interesting in my opinion. In the archaeological supplementary it is noted that at one of these sites they uncovered more than a 117 skeletons. They tested a total of 6 samples from 3 sites however.

All in all a very interesting article, with fascinating data. I just wished they also included later samples in their genome-wide analysis, because we know they exist!

So why the title?

I've seen tons of people make statements about "The Tarim Mummies", as they were a set population. They are not. These mummies are a natural phenomenon due to the unique climatic conditions of the Taklamakan desert, which allows for the exceptional preservation of organic materials that we all know of. If I were to die in the desert I could eventually end up as one. Even worse are the inferences made regarding Tocharian speakers, as if we have any evidence that these early mummies were of Tocharian speakers. Or any of the mummies for that matter.

Some of these Tarim mummies date to as early as 1800 b.c, others date to the 4th century A.D, a timespan of two millennia. Several of the famous ones such as Cherchen man and his two wives, or the Subeshi witches are not from the bronze age, but the iron age.

In this study, they have looked at the 1800-1500 b.c mummies of the Xiaohe, Gumugou and Beifang Mudi / Ayala Mazar, the latter name meaning cemetery of women referring to it's high share of female burials, which are grouped in together as part of the same material culture based on the similar traditions, items, clothing, and physical characteristics of the populations.

Speaking of physical characteristics, I'm mostly writing about this ancient DNA article as an excuse to post mummies anyways so here you go:


Mummies from the Beifang Mudi/Ayala Mazar site


The beauty of Loulan


The Xiaohe princess and other mummies from the Xiaohe cemetery



In the iron age, these material cultures ceased to exist and you had other ones, which also had their fair share of mummies. One  particularcluster are the sites of Yanghai, Subeixi and Aidinghu which are considered to be of shared tradition as well. Given their presence in the Turfan oasis during the early iron age they might be prime candidates for early Tocharian speakers in my opinion, but it remains to be seen if they were.


The mummy of the 40-year-old horse rider from the Yanghai cemetery, Turfan Oasis (M21)

The 'Shaman' from the Yanghai cemetery, Turfan Oasis (M80)

Reconstruction of the Yanghai shaman grave

The Yanghai site was recently featured in a wonderful documentary by Survive The Jive about the usage of Cannabis amongst ancient Indo-Europeans. Great video you should watch it if you haven't:


The famous Cherchen man or Ur-David, from the early iron age Zaghunluq/Zahonluke cemetery in the southeastern part of the Tarim Basin and it is in the region where 'Tocharian C' presumably was spoken during the iron age. Does that mean that Cherchen man was a Tocharian speaker in life? Perhaps, but we cannot know for sure. In my opinion these are the most stunning finds of the Tarim Basin, and the ones which really set of that whole mystery for me. The symbols, clothing, tattoos and hairstyles culminate in a very aesthetically pleasing package. 







Cherchen man and the two Cherchen women

Another one of my archaeological favorites is The Yingpan man. This mummy is from the third or fourth century A.D, found upstream of the Konque river to the west of Xiaohe, and might have been a Sogdian merchant. Based on his very lavish and expensive materials from distant regions you can assume he was a very wealthy man. His caftan has graeco-roman designs on it and the materials probably hailed fom the west. Lots of gold on his clothing, including the shoes. Tall man as well with a height above 190 cm. The Sogdian P.I.M.P of the Silk Road ( I will make a post about that as well).




I think this is a good amount of mummies for now. I can post more eventually however. I'll be dropping more of my musings and findings regarding the samples in here. 

10 comments:

  1. "I have to say that I wasn't impressed with their conclusions for the Siberian and Central Asian populations derived from Neolithic West-Siberian related peoples."

    To elaborate, I recreated the proposed ancestry components of these samples with the ones mentioned in the article:

    Target: KAZ_Mereke_MBA
    Distance: 2.7712% / 0.02771155
    71.8 CHN_Dzungaria_EBA1
    28.2 CHN_Tarim_EMBA1

    Target: KAZ_Kumsay_EBA
    Distance: 1.9026% / 0.01902603
    50.8 CHN_Tarim_EMBA1
    42.6 RUS_Afanasievo
    6.6 TKM_Geoksyur_En

    Versus:

    Target: KAZ_Kumsay_EBA
    Distance: 1.3209% / 0.01320949
    49.4 RUS_Progress_En
    37.0 CHN_Tarim_EMBA1
    12.4 RUS_Sosonivoy_HG
    1.0 TKM_Geoksyur_En
    0.2 RUS_Afanasievo

    Target: KAZ_Mereke_MBA
    Distance: 1.5566% / 0.01556643
    34.8 RUS_Progress_En
    24.4 RUS_Sosonivoy_HG
    23.2 CHN_Tarim_EMBA1
    12.6 CHN_Dzungaria_EBA1
    5.0 TKM_Geoksyur_En


    Target: KAZ_Mereke_MBA
    Distance: 1.4187% / 0.01418669
    34.2 CHN_Tarim_EMBA1
    23.6 RUS_Poltavka
    18.8 RUS_Progress_En
    16.6 RUS_Sosonivoy_HG
    6.8 TKM_Geoksyur_En
    0.0 CHN_Dzungaria_EBA1

    Obviously I'm using a bit more sources here but the exclusion of Sosonivoy/Tyumen doesn't change things all that much. The models with Progress_EN are more appropriate in terms of genetic proximity, and also fit better with the archaeological record.

    Also, are they implying that eneolithic southern Central Asians were majority Anatolian neolithic? Crazy to even put that out there. I assume it's just a mistake in their map.

    Target: TKM_Geoksyur_En
    Distance: 4.2442% / 0.04244215
    75.8 IRN_Ganj_Dareh_N
    12.8 TUR_Kumtepe_N
    11.4 CHN_Tarim_EMBA1

    But adding something like Kumsay_EBA, which has that Progress/Vonyuchka type ancestry, The is no necessity for "pure" Tarim_EMBA ancestry anymore:

    Target: TKM_Geoksyur_En
    Distance: 3.5225% / 0.03522505
    73.0 IRN_Ganj_Dareh_N
    17.0 KAZ_Kumsay_EBA
    10.0 TUR_Kumtepe_N
    0.0 CHN_Tarim_EMBA1

    My bet is that because people like the Kelteminar were more similar to Kumsay types than Tarim_EMBA types. It's a consistent pattern I have seen with southern central asian samples that all their WSHG-related ancestry also has some Steppe_EN.

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  2. Yes, finally a centralized place to get Copper Axe's insights! Why is that you write in English better than me if it is your second language?

    Weaved baskets? So if not for mummification we would even know they had containers for things.

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  3. @Genos

    Welcome!

    Yeah weaving baskets are apparently much older than pottery, and you have examples from more than 20k years ago of hunter-gatherers making baskets.

    Question, what did you make of the snps in the article? This is one of the rare times where we can connect ancient genotypes to ancient phenotypes, atlhough you always have to take care with remains as aged as those. For the Tarimians, the light variants of rs1426654 and rs16891982 were lacking, but it seems like there are some calls for M1cr and KITLG snps responsible for hair depigmentation and/or an increased chance of it. I think rs1800404 has an association with depigmentation amongst East Asian peoples, but I have no clue to which degree. They didn't look for the primary snps responsible for East Asian pigmentation which is a bit of a bummer because they seem to have some mutations which are generally quite East Asian, and therefore could have had other ones.

    Another weird thing is this sample here, from Shirenzigou:

    Target: CHN_Shirenzigou_IA:M15-1
    Distance: 4.4569% / 0.04456949
    41.0 KAZ_Kumsay_EBA
    18.8 MNG_Ulaanzuukh_Slab_Grave
    16.6 IRN_Shahr_I_Sokhta_BA2
    15.0 RUS_Sintashta_MLBA
    7.8 UZB_Bustan_BA
    0.8 CHN_Tarim_EMBA1

    So I already knew this sample had an absurdly high amount of ANE ancestry for it's age (300-100 bc), but the crazy part is that G25 doesn't show it as coming from Tarim_EMBA type populations but rather the Kumsay/Steppe Maykop variant. And the individual likely was recently admixed with people from the steppes as well, so there should be a recent ancestor which primarily had this indigenous central asian profile.

    The Tarim basin really was a magical place, home to all sort of fantastical creatures. Orcs and Goblins...

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  4. Great write up. Puts everything in perspective. I seriously doubt they resisted geneflow from IE neighbours beyond 1000 BC

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  5. Ok, yeah I see the Xiaohe mummies do have some light skin genes. I've studied some of these SNPs. I have an idea of their possible impact.

    All have light skin allele in rs1804004 of OCA2 gene. This is Eurasian, African-wide SNP.

    A few have light skin allele in rs885479 in MC1R gene which seems to be main identified light skin gene in East Asia.

    rs1804004 is human-wide it exists in Africa & Eurasia.

    A study on skin color in Africa, named it as one of the most influential SNPs, as influential as rs1426654.

    What is interesting is light skinned Khoisan have a really high frequency but all other Africans have a really low frequcny. It along with a few other SNPs can explain Khosian light skin.

    Yeah, I guess this could suggest light skin tone in Tarim Mummies. I'd be interested to look at rs1804004 in other ancient North Asians. Maybe they developed light skin independently by selecting this SNP and East Asian SNPs.

    But maybe not. It's not possible to determine skin color for an extinct people.

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  6. That's crazy. I have to look at the M15 sample in Shirenzigou. The fit is high (@4.4) so he must be low coverage. None the less it should be able to accurately say whether he has Steppe-Maykop-related stuff instead of Sintashta mixed with "ANE" [indigenous Central Asians, whatever :)].

    Ancient DNA always bring sup these surprises. In one sense it is fascinating, in one sense it is confusing.

    Steppe Maykop-related didn't even live that far east in Asia. So what would a mostly Steppe Maykop-relate person be doing in western China 2,000 years after they supposedly disappeared? This doesn't sound right.

    I'll look at the sample. I haven't even looked at the Shirenzigou Iron age samples from Western China yet. I was surprised from the beginning when i heard people say they had lots of ANE and R1b2. You're right it was a precursor to these Tarim Mummies.

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  8. Amazing article and hope we see more posts of you here. The Tarim Basin is some magical place i somehow feel very attached for. An amazing symbiosis of Tocharians, Iranics, Indo-Aryans, native Pre-IE Central Asians, Turkics and other people. These new tarim basin genomes are fantastic too and again showing us mysterious people we not even were aware of existing and dying out. This is the beauty of ancient dna it is bringing back to history and human consciousness long forgotten ancient people.

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  9. I dont want to clutter up my own comment section with my long ass rambles so I will do a 3 for 1 combo deal:

    @Coldmountains Thanks! I agree with you 100% brother, its easily one of my favorite regions in term of history and culture, I'll be sure to post more about it.

    One cool thing about Central Asia + adjacent regions is that in term of the massive Indo-European migrations it all happened a bit later we get to witness the native populations develop a bit more, which in terms of ancient DNA has been reflected well so we have many of these ANE-rich samples which are related, but still all different such as Botai, Okunev, Kumsay, Aigyrzhal etc. Over here in Europe its all much more simpler in terms of ancient population genetic profiles.

    The western steppe herders are a fascinating case of foragers adapting agriculture and metalworking from advanced farmers and using it to effectively spread across their native habitat and beyond, changing the course of history forever. But in Central asia and Siberia, we see this happen many times with the various native peoples, with IE peoples often playing the role neolithic farmers had with their ancestors in terms of technological and cultural diffusion.

    Seima Turbino is a good example with metalworking techniques from south Central Asia reaching Dzungaria and the Altai , from there spreading across Siberia and reaching the Urals and Eastern Europe.


    @Survive The Jive

    Thanks man! For sure, even going by linguistics Tocharian should have began to separate by then and unless there were three separate migrations into the eastern Tarim, Tocharians at least had to be in the vicinity. 1500-1000 bc is probably the timeframe when the action takes place in the eastern tarim in terms of IE migrations.

    @Genos Historia

    Yeah its odd right?

    Note that Xinjiang although part of China effectively is Central Asia, and just over the mountains west of Xinjiang prior to the great expansion of the Indo-Iranians you had a whole mishmash of Botai-like, Kumsay-like and BMAC/Helmand related ancestries flowing about.

    My bet is that this steppe-maykop/kumsay profile is also closely related to the Kelteminar and the like, and that this profile formed somewhere in the Ural-Caspian, and then spread across both sides of the Caspian shores in the neolithic/eneolithic.

    That type of Kumsay ancestry is currently the best working proxy for the WSHG ancestry in the BMAC samples, and that modern of South Central and South Asians, although mediated through a significantly Central Asian farmer admixed meta-population.

    So in the bronze age you had this ancestry all over Central Asia, especially as pastoralists along the central asian mountain ranges, with Xinjiang on the other side. Did some of these populations manage to last somewhere deep in the mountains until the iron age? Strange indeed, but not any less plausible than "isolated since the mesolithic" ice age ANE remnants suriving in the taklamakan desert.

    Most of those Shirenzigou samples are of pretty poor quality, but you have those Western Xiongnu samples from Damgaard 2018 which are basically identical to the 'regular' samples.

    I will be making a post about the Kumsay site soon, it will cover some of the physical anthropology as well. This is definitely something you want to cover in the collab mate. I'm not gonna spoil too much but we have found our Uruk-hai. In an alternative universe we would've been their descendants!

    Also anyone who would find calling an extinct race Orcs to be offensive or racist can go take a long hike and never return lmao. Its too good of a name not to use. I recently found out Orc has become a widespread slang word in Mongolia for rural meatheads. Awesome people man bless them.

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  10. The Chiricahua Apaches speak Tartar Chinese.

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